Anthony Kossiakoff, PhD

The Kossiakoff Group's research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful that traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.

California Institute of Technology
Postdoc - Structural Biology
1975

University of Delaware
Ph.D - Physical Chemistry
1972

Davis and Elkins College
BS - Chemistry/Math
1968

Structures of ABCB4 provide insight into phosphatidylcholine translocation.
Nosol K, Bang-Sørensen R, Irobalieva RN, Erramilli SK, Stieger B, Kossiakoff AA, Locher KP. Structures of ABCB4 provide insight into phosphatidylcholine translocation. Proc Natl Acad Sci U S A. 2021 Aug 17; 118(33).
PMID: 34385322

Structure of an AMPK complex in an inactive, ATP-bound state.
Yan Y, Mukherjee S, Harikumar KG, Strutzenberg TS, Zhou XE, Suino-Powell K, Xu TH, Sheldon RD, Lamp J, Brunzelle JS, Radziwon K, Ellis A, Novick SJ, Vega IE, Jones RG, Miller LJ, Xu HE, Griffin PR, Kossiakoff AA, Melcher K. Structure of an AMPK complex in an inactive, ATP-bound state. Science. 2021 07 23; 373(6553):413-419.
PMID: 34437114

Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1.
Chen Q, Plasencia M, Li Z, Mukherjee S, Patra D, Chen CL, Klose T, Yao XQ, Kossiakoff AA, Chang L, Andrews PC, Tesmer JJG. Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1. Nature. 2021 Jul; 595(7868):600-605.
PMID: 34262173

K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle.
Yu Y, Zheng Q, Erramilli SK, Pan M, Park S, Xie Y, Li J, Fei J, Kossiakoff AA, Liu L, Zhao M. K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle. Nat Chem Biol. 2021 08; 17(8):896-905.
PMID: 34239127

Structural basis of omega-3 fatty acid transport across the blood-brain barrier.
Cater RJ, Chua GL, Erramilli SK, Keener JE, Choy BC, Tokarz P, Chin CF, Quek DQY, Kloss B, Pepe JG, Parisi G, Wong BH, Clarke OB, Marty MT, Kossiakoff AA, Khelashvili G, Silver DL, Mancia F. Structural basis of omega-3 fatty acid transport across the blood-brain barrier. Nature. 2021 07; 595(7866):315-319.
PMID: 34135507

Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody that Targets two Distinct Epitopes on av Integrins.
Gallo E, Kelil A, Haughey M, Cazares-Olivera M, Yates BP, Zhang M, Wang NY, Blazer L, Carderelli L, Adams JJ, Kossiakoff AA, Wells JA, Xie W, Sidhu SS. Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody that Targets two Distinct Epitopes on av Integrins. J Mol Biol. 2021 07 23; 433(15):167090.
PMID: 34090922

Engineered Ultra-High Affinity Synthetic Antibodies for SARS-CoV-2 Neutralization and Detection.
Slezak T, Kossiakoff AA. Engineered Ultra-High Affinity Synthetic Antibodies for SARS-CoV-2 Neutralization and Detection. J Mol Biol. 2021 05 14; 433(10):166956.
PMID: 33775667

A T cell redirection platform for co-targeting dual antigens on solid tumors.
Enderle L, Shalaby KH, Gorelik M, Weiss A, Blazer LL, Paduch M, Cardarelli L, Kossiakoff A, Adams JJ, Sidhu SS. A T cell redirection platform for co-targeting dual antigens on solid tumors. MAbs. 2021 Jan-Dec; 13(1):1933690.
PMID: 34190031

Recognition of an a-helical hairpin in P22 large terminase by a synthetic antibody fragment.
Lokareddy RK, Ko YH, Hong N, Doll SG, Paduch M, Niederweis M, Kossiakoff AA, Cingolani G. Recognition of an a-helical hairpin in P22 large terminase by a synthetic antibody fragment. Acta Crystallogr D Struct Biol. 2020 Sep 01; 76(Pt 9):876-888.
PMID: 32876063

Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling.
Tsutsumi N, Mukherjee S, Waghray D, Janda CY, Jude KM, Miao Y, Burg JS, Aduri NG, Kossiakoff AA, Gati C, Garcia KC. Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling. Elife. 2020 08 07; 9.
PMID: 32762848

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Christian Anfinsen Award
Protein Society
2019

Fellow of American Association for Advancement of Science
AAAS
2012

Honorary Degree Doctor of Science
Davis and Elkins College
2011

Alumni Hall of Fame
University of Delaware
2003

Chair Department of Biochemistry and Molecular Biology
University of Chicago
1998 - 2011

Director Institute for Biophysical Dynamics
University of Chicago
1998 - 2003

Director Department of Protein Engineering
Genentech, Inc.
1983 - 1998