Anthony Kossiakoff, PhD

The Kossiakoff Group's research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful that traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.

California Institute of Technology
Postdoc - Structural Biology
1975

University of Delaware
Ph.D - Physical Chemistry
1972

Davis and Elkins College
BS - Chemistry/Math
1968

Recognition of an a-helical hairpin in P22 large terminase by a synthetic antibody fragment.
Lokareddy RK, Ko YH, Hong N, Doll SG, Paduch M, Niederweis M, Kossiakoff AA, Cingolani G. Recognition of an a-helical hairpin in P22 large terminase by a synthetic antibody fragment. Acta Crystallogr D Struct Biol. 2020 Sep 01; 76(Pt 9):876-888.
PMID: 32876063

Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling.
Tsutsumi N, Mukherjee S, Waghray D, Janda CY, Jude KM, Miao Y, Burg JS, Aduri NG, Kossiakoff AA, Gati C, Garcia KC. Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling. Elife. 2020 08 07; 9.
PMID: 32762848

Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins.
Mukherjee S, Erramilli SK, Ammirati M, Alvarez FJD, Fennell KF, Purdy MD, Skrobek BM, Radziwon K, Coukos J, Kang Y, Dutka P, Gao X, Qiu X, Yeager M, Eric Xu H, Han S, Kossiakoff AA. Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins. Nat Commun. 2020 03 27; 11(1):1598.
PMID: 32221310

Structure and mechanism of the ER-based glucosyltransferase ALG6.
Bloch JS, Pesciullesi G, Boilevin J, Nosol K, Irobalieva RN, Darbre T, Aebi M, Kossiakoff AA, Reymond JL, Locher KP. Structure and mechanism of the ER-based glucosyltransferase ALG6. Nature. 2020 03; 579(7799):443-447.
PMID: 32103179

Structure and drug resistance of the Plasmodium falciparum transporter PfCRT.
Kim J, Tan YZ, Wicht KJ, Erramilli SK, Dhingra SK, Okombo J, Vendome J, Hagenah LM, Giacometti SI, Warren AL, Nosol K, Roepe PD, Potter CS, Carragher B, Kossiakoff AA, Quick M, Fidock DA, Mancia F. Structure and drug resistance of the Plasmodium falciparum transporter PfCRT. Nature. 2019 12; 576(7786):315-320.
PMID: 31776516

An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability.
Slezak T, Bailey LJ, Jaskolowski M, Nahotko DA, Filippova EV, Davydova EK, Kossiakoff AA. An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability. Protein Sci. 2020 01; 29(1):141-156.
PMID: 31622515

Development of "Plug and Play" Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM.
Dutka P, Mukherjee S, Gao X, Kang Y, de Waal PW, Wang L, Zhuang Y, Melcher K, Zhang C, Xu HE, Kossiakoff AA. Development of "Plug and Play" Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM. Structure. 2019 12 03; 27(12):1862-1874.e7.
PMID: 31669042

Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin.
Farcasanu M, Wang AG, Uchanski T, Bailey LJ, Yue J, Chen Z, Wu X, Kossiakoff A, Tang WJ. Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin. Biochemistry. 2019 07 09; 58(27):2996-3004.
PMID: 31243996

Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2.
Sun J, Paduch M, Kim SA, Kramer RM, Barrios AF, Lu V, Luke J, Usatyuk S, Kossiakoff AA, Tan S. Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2. Proc Natl Acad Sci U S A. 2018 10 02; 115(40):10010-10015.
PMID: 30224453

Structural basis for activation of voltage sensor domains in an ion channel TPC1.
Kintzer AF, Green EM, Dominik PK, Bridges M, Armache JP, Deneka D, Kim SS, Hubbell W, Kossiakoff AA, Cheng Y, Stroud RM. Structural basis for activation of voltage sensor domains in an ion channel TPC1. Proc Natl Acad Sci U S A. 2018 09 25; 115(39):E9095-E9104.
PMID: 30190435

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Christian Anfinsen Award
Protein Society
2019

Fellow of American Association for Advancement of Science
AAAS
2012

Honorary Degree Doctor of Science
Davis and Elkins College
2011

Alumni Hall of Fame
University of Delaware
2003

Chair Department of Biochemistry and Molecular Biology
University of Chicago
1998 - 2011

Director Institute for Biophysical Dynamics
University of Chicago
1998 - 2003

Director Department of Protein Engineering
Genentech, Inc.
1983 - 1998